Hello everyone,

I want to know what should be the optimum incubation time for ligation? In our lab, we are using Thermo Scientific™ Rapid DNA Ligation Kit Catalog number: K1422 for ligation, the datasheet of the respective kit says to incubate the reaction mix at 22°C for 5 min. Although I have done ligation followed by transformation once at 22°C for 1hr as suggested by our senior members. But there were no transformed colonies?