What should be preferred, using proteinase K and declocker for antigen retrieval in IHC?

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Frazer Bell

Yes there is a difference, each antibody you use has to be optimised, both antigen retrieval and concentration of primary antibody etc...

You should consult the spec sheet for the antibody as a starting point.

Most antibodies require heat induced epitope retrieval HIER using a pH buffer usually sodium citrate pH6 or EDTA pH8 or 9.

If you use proteinase k you will need to optimise the digestion time.

Seda Keskin

Dear Doctor Iqbal,

This article may solve your problem..

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749998/

Good luck👍