What should be considered in the application of pfu enzyme instead of Taq polymerase in PCR?
Dear researchers; I am about to simulation a drop test that a 4kg ball drops a from500cm height on my sample. In my experimental test, our sample doesn't damage anymore. but when I simulate it in...
28 June 2024 9,925 6 View
Art-based approaches to Peace Education, Human Rights Promotion & Social Justice Cultivation are now an almost new trend. Can activities through online media be considered in this field?
16 March 2024 6,155 3 View
If you are a clinical librarian, please answer! A. To what extent is your knowledge used in medical science research projects? 1. Very Frequently 2. Frequently 3. Occasionally 4. Rarely...
28 October 2023 1,713 2 View
In reading classes work on book content using action verbs of six levels of Bloom s taxonomy and also the technique of Q and A in such classes? As Benjamin Bloom claims that it works well in...
15 October 2023 4,166 2 View
What is the importance of a delayed posttest in language teaching research? When should it be done? After or before the post test?
12 October 2023 1,908 3 View
What is the best way to freeze-dry (lyophilise) the extracted DNA? we need high quality for NGS
23 October 2021 7,072 5 View
How can we distinguish Bifidobacterium strains from colony appearance and gram staining or culture condition? B. bereve B. bifidum B. longum B. lactis
29 September 2021 2,434 1 View
Hello dear researchers. I run the siamfc ++ algorithm. It showed better results than the other algorithms but its accuracy is still not acceptable. One of my ideas is to run an object detection...
06 September 2021 4,928 3 View
Which object tracking algorithms are highly accurate and also have high processing speed? Opencv algorithms themselves do not work well at high speeds Thank you for your help
07 August 2021 8,747 3 View
for Identification of Bifidobacterium stains we need pure genomic DNA for 16s rRNA PCR and sequencing
28 June 2021 1,863 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View