Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Can Qualtrics generate reports for survey respondents that show their answers to selected questions?

I am using Qualtrics for a Delphi survey. Does anyone know if Qualtrics can generate reports that I can send to respondents. More specifically, I want to send respondents a report of their...

13 January 2021 7,976 3 View

What are health and agricultural econmic impact of the herbicide 2,4-dichlorophenoxyacetic_acid (2,4-D) in your country?

Dear Research community, I am writing a review about 2,4-D. Google Scholar has identified 11000 publications with this herbicide as a keyword since 01/01/2018. There is plenty for me to read....

29 December 2020 1,145 6 View

What are the ethical considerations when conducting research with students who are paying for a private course?

I'm going to be teaching an online course to minors using game-based learning strategies. I wasn't to make sure I consider ethics considering students (likely their parents) will be paying to take...

12 December 2020 6,497 11 View

No host reference for metatranscriptomics?

One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...

30 November 2020 3,259 2 View

Primer specificity check with primer blast individually or together?

Hi, When checking my designed primers for specificity, what is the difference if I run BLASTn for each primer individually versus together (ie. forward primer separated by 20 "N" then reverse...

03 November 2020 1,421 4 View

To what extent do motorhomes and other large vehicles degrade road surfaces in relation to cars and other light vehicles?

I am studying the effects on the road system of a rural region of a drastic increase in traffic, particularly Motorhomes. My question is this, do motorhomes and other large vehicles have a...

22 October 2020 8,146 3 View

How to calculate pKa's using DFT for a molecule with three acidic hydrogens?

The molecule in question is a molecule with 3 non-equivalent hydrogens. Am I going to have to run the calculations in all of the transition states?

18 October 2020 1,524 3 View

Gibson Assembly Produces Wrong Product?

I have been using Gibson Assembly to insert a 7kB fragment into an 8kB linearized vector. After assembly many colonies form, but colony PCR on the transformation consistently shows a 1.5 kB...

18 September 2020 247 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View