When making gels from polyacrylamide, what kind of properties of the gel I should monitor in order to make sure that I am consistently producing the same every time? Any suggestions? Thank you
I know enzyme catalyzed reactions and Ti(OBu)4 catalyzed reactions make such polyesters with siloxanes (PDMS), but are there any other methods?
04 May 2013 488 0 View
I have a material containing coumarin in it. When my collaborator takes fluorescence microscopic images of the material using DAPI filter, he mentioned me that the sample is photobleaching. As a...
31 December 2012 1,677 11 View
I am looking for photodegradable polymers which are commercially available and cheap. Please let me know if you are aware of such polymers.
31 December 2012 9,028 2 View
The monomer (di alcohol) is not soluble in chloroform or dichloromethane, and the solubility of di-isocyanate is not an issue. I am using tin octonate as catalyst.
11 December 2012 3,223 2 View
Applications of photoreversible systems
01 February 2012 737 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.
09 August 2024 2,111 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...
24 July 2024 5,908 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View