When making gels from polyacrylamide, what kind of properties of the gel I should monitor in order to make sure that I am consistently producing the same every time? Any suggestions? Thank you
I know enzyme catalyzed reactions and Ti(OBu)4 catalyzed reactions make such polyesters with siloxanes (PDMS), but are there any other methods?
04 May 2013 401 0 View
I have a material containing coumarin in it. When my collaborator takes fluorescence microscopic images of the material using DAPI filter, he mentioned me that the sample is photobleaching. As a...
31 December 2012 1,592 11 View
I am looking for photodegradable polymers which are commercially available and cheap. Please let me know if you are aware of such polymers.
31 December 2012 8,949 2 View
The monomer (di alcohol) is not soluble in chloroform or dichloromethane, and the solubility of di-isocyanate is not an issue. I am using tin octonate as catalyst.
11 December 2012 3,148 2 View
Applications of photoreversible systems
01 February 2012 647 1 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...
25 February 2021 8,053 5 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View