Hi,

There are many advanced programs available for single particle reconstruction., To start with, I hope Eman 2 , Relion serves your purpose! Xmipp is also an interesting tool to consider!

Thank you. Did you use relion

I use Eman 2 quite often! not Re-lion.., But heard from my colleagues that relion is comparatively better for final stages of refinement! I will definitely use it n future! 

There is a whole list of available software packages:http://www.emdatabank.org/emsoftware.html

And for an icosahedral case, almost any of them would work well

Bsoft

http://lsbr.niams.nih.gov/bsoft/bsoft.html

Cyclops

http://www.bfsc.leidenuniv.nl/software/Cyclops/

CryoSPARC

http://cryosparc.com

EMAN

http://blake.bcm.edu/EMAN/

Eos

http://www.yasunaga-lab.bio.kyutech.ac.jp/Eos/

IMAGIC

http://www.imagescience.de/imagic/index.htm

IPLT

http://www.iplt.org/wiki

MDPP

http://gcrc.med.nyu.edu/mdpp/

MRC

http://www.ccpem.ac.uk/download.php

Simple

http://simplecryoem.com/

Sparx

http://macro-em.org/sparxwiki/SparxWiki

Spider

http://www.wadsworth.org/spider_doc/spider/docs/spider.html

Suprim

http://emg.nysbc.org/redmine/projects/suprim

SPHIRE

http://sphire.mpg.de/

Xmipp

http://xmipp.cnb.csic.es/

Thank you  for information. which program is suitable for cryo-em model fitting into electron density

Hi Govindasamy Lakshmanan , Please use Phenix suite which is supported by linux, MacOS and also windows. It has now integrated the workflow for cryoEM including real space refinement, map sharpening, validation tools, etc.

Also, phenix is much faster than its counterparts in my experience.