Why do I keep getting smudged and wavy bands in certain lanes of my western blots?

Hi everyone, Been doing western blots for years now, recently however, whenever I run 2 gels, one of the gels would have a few lanes that exhibit wavy or smudgy bands at around 50 kDa (see...

16 November 2020 3,759 11 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Data and modeling for vapor-liquid metastable states, spinodals?

What publications are available with actual measured data on vapor-liquid metastable states and spinodals and the modeling thereof?

16 February 2020 9,000 1 View

I seem to have Protein stuck on my C8 column. How do I clean it properly?

Hi everyone, I've been trying to detect a Peptide Hormone (ca. 9 kDa) by RP-HPLC using H2O + 0,1% TFA and MeOH + 0,1% TFA as mobile phases with a flow rate of 1 mL/min, a C8 column with a...

07 April 2019 3,053 11 View

In Black hole theory, have mathematicians finally lost all connection with physics?

18 November 2018 4,730 38 View

Model Implied Instrumental Variables experiences?

MIIV (http://fgme2017.de/kenneth-bollen/) looks like a very promising approach. Are there any experiences from research practice? Application-oriented publications? When is it appropriate to use...

09 February 2018 174 1 View

Which is the best culture media for culturing macrophages?

08 October 2014 3,945 19 View

Is anyone familiar with In-vitro RNA transcription for long >6 kb transcripts?

20 September 2014 2,928 2 View

What kind of statistical test should I choose if I want to test regression to the mean on related data set?

I conducted an experiment to investigate the effect of a physical therapy treatment on the signal intensities of the 5 lumbar discs (L1-2 to L5-S1) on MR images. I found that the signal intensity...

07 March 2014 4,762 6 View

Has the speed of light been accurately measured in space, and would it be the same as on earth?

I believe there is reason to expect that the measure of the speed of light on the International Space Station would be greater than it is measured at the surface of the earth. My expectation is...

10 November 2013 2,652 32 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View