Why do I keep getting smudged and wavy bands in certain lanes of my western blots?

Hi everyone, Been doing western blots for years now, recently however, whenever I run 2 gels, one of the gels would have a few lanes that exhibit wavy or smudgy bands at around 50 kDa (see...

16 November 2020 3,759 11 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Data and modeling for vapor-liquid metastable states, spinodals?

What publications are available with actual measured data on vapor-liquid metastable states and spinodals and the modeling thereof?

16 February 2020 9,000 1 View

I seem to have Protein stuck on my C8 column. How do I clean it properly?

Hi everyone, I've been trying to detect a Peptide Hormone (ca. 9 kDa) by RP-HPLC using H2O + 0,1% TFA and MeOH + 0,1% TFA as mobile phases with a flow rate of 1 mL/min, a C8 column with a...

07 April 2019 3,053 11 View

In Black hole theory, have mathematicians finally lost all connection with physics?

18 November 2018 4,730 38 View

Model Implied Instrumental Variables experiences?

MIIV (http://fgme2017.de/kenneth-bollen/) looks like a very promising approach. Are there any experiences from research practice? Application-oriented publications? When is it appropriate to use...

09 February 2018 174 1 View

Which is the best culture media for culturing macrophages?

08 October 2014 3,945 19 View

Is anyone familiar with In-vitro RNA transcription for long >6 kb transcripts?

20 September 2014 2,928 2 View

What kind of statistical test should I choose if I want to test regression to the mean on related data set?

I conducted an experiment to investigate the effect of a physical therapy treatment on the signal intensities of the 5 lumbar discs (L1-2 to L5-S1) on MR images. I found that the signal intensity...

07 March 2014 4,762 6 View

Has the speed of light been accurately measured in space, and would it be the same as on earth?

I believe there is reason to expect that the measure of the speed of light on the International Space Station would be greater than it is measured at the surface of the earth. My expectation is...

10 November 2013 2,652 32 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View