I am interested in labeling Lactobacillus plantarum with either a red (preferably) or a green fluorescent protein. Can anyone recommend a paper that describes a molecular tool for doing this?
Thank you for your help.
Hi Mark,
The authors of this paper may be able to provide you with one of their constructs, if not how to make them and the vectors to start with are well described.
Article Use of green fluorescent protein to monitor Lactobacillus pl...
Frank
Thank you, Frank. I did see this paper before; I will get in touch with them.
Here is a paper that discusses the use of infrared fluorescent protein (IRFP) for tracking lactic acid bacteria (LAB) in the mouse GI tract. Not sure if I can use it in flies, but in case anyone else is interested!
Article In vivo imaging of Lactococcus lactis, Lactobacillus plantar...
I also found a 2021 paper that seems promising!
Article Persistence and dynamics of fluorescent Lactobacillus planta...
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