What percentages of CD4+ or CD8+ T cells are Tbet positive in spleen?

More Daxing Gao's questions See All

How would I calculated ddPCR spiked nucleic acid recovery calculations?

Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...

02 July 2024 6,011 2 View

Does the zirconium isotope fractionate as through the column?

Is the zirconium isotope of geological sample fractionated when passing through the column, and how to determine whether this fractionation is or not？

26 May 2024 6,721 2 View

In C++, why is the constructor of a virtual base class called first, while the object of the virtual base class is placed last?

In C++, why is the constructor of a virtual base class called first, while the object of the virtual base class is placed last? What are the advantages of doing this?

07 May 2024 1,583 1 View

Through what theories can we aptly explain the social networks and subcultures that people form around cats?

Just like the stray cat protection organizations on campus, they are spontaneous and not organized by the school. They provide a series of services for kittens in life and death. And the source of...

11 April 2024 4,944 0 View

PMSCV for protein transit protein expression?

We are using pMSCV for transit expression of a protein.: 1. The gene was cloned in (between XhoI/EcoRI). 2. Transfection with lipofectmin 3000 to 293t cell. 3. After 48 hrs, GFP can be...

04 April 2024 8,350 4 View

How to download the protein sequences included in the CAZy databases?

In the latest CAZy sequence list (http://www.cazy.org/), over 3,600,000 sequecnes have NCBI accession and they can be easily accessed. However, the remaining 550,000 sequences only have a jgi...

13 March 2024 2,439 1 View

Who want to publish papers to Scopus journal?

Computer Systems Science and Engineering ISSN:0267-6192(print) Publish within 4 months Low APC: 600 USD(Review paper full waiver) Send me DM for more info

31 January 2024 5,463 1 View

How to calculate the localization length in 1d optical random system?

In 1D random strustures, the Anderson localization is an important phonomenon. Localization length is a key parameter to show the localization characteristic. How to calculate the localization...

28 December 2023 3,999 1 View

How to program spatially correlated Rician fading channels in the RIS enhanced MIMO networks with Matlab?

Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance the...

08 December 2023 6,883 4 View

Spatially correlated Rician fading channels in the IRS Enhanced MIMO Networks?

Hi! I have questions to consult with scholars. We consider a multiuser MISO downlink communication system where an IRS equipped with a UPA ( with M reflecting elements) is deployed to enhance...

07 December 2023 7,563 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View