Daxing Gao

6 Questions 6 Answers 0 Followers

Questions related from Daxing Gao

What are the CD11b+F4/80-Ly6G-CD115- cells?

CD11b+F4/80-Ly6G-CD115+should be monocytes. But what about the CD115- population? Granulocyte-Monocyte Progenitors and Monocyte-Dendritic Cell... In this paper, Fig1. Both GMP and MDP can...

12 October 2021 9,086 0 View

Could anyone suggest a protocol of immunohistochemistry for CD4 and CD8 in mouse paraffinized tissue?

Does anybody have a brief protocol or experience for CD4/CD8 IHC in mouse tissue? I'm using biotinylated-antibody from biolegend. I want to know the method for antigen retrieval (Heat Induced...

11 August 2014 8,269 18 View

What percentages of CD4+ or CD8+ T cells are Tbet positive in spleen?

I am trying to stain mouse spleen cells for Tbet. I wonder what percentages of CD4+ T cells or CD8+ T cells are Tbet positive in spleen of wild type B6 mice? Thanks in advance!

26 July 2014 9,777 3 View

Anyone have experience with mouse serum IL-12 ELISA?

Recently I have been working with detecting serum IL12 by ELISA. Based on that result, the sera from healthy mice have around 10000~20000pg/mL IL12p40/p70. But when I searched the literature,...

30 June 2014 3,095 2 View

How to give a mouse intra articular injection of TNF to induce arthritis?

Does anybody have the procedure for intra articular injection? And how much TNF should be injected? Thanks!

01 April 2014 2,128 1 View

What's the CD11b-LyC(hi) population in mouse PBMCs?

When I stained mouse PBMCs, I saw there was a population of Ly6c+CD11b- cells (>10%) and these cells express CD86. From the FSC and SSC, I can tell they are not lymphocytes. Does anybody have an...

28 February 2014 6,559 6 View