What percentage of stacking and resolving gel is required to run a protein of molecular weight 400 kDa?

04 April 2018 1 491 Report

I have a protein of molecular mass 400 kDa? What is the required protein gel concentration for both stacking and resolving gel?

Prarthna Saraswat

Generally To resolve a protein of molecular weight 400KDa, 7% Resolving SDS-PAGE Gel is sufficient. stacking gel percentage is 5%.

7% Resolving gel can resolve proteins with molecular weight ranging 50KDa-500KDa.

Hope it helps.

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