What must be the Classification (particle count) and negative pressure for virus lab for clinical grade production of viral vectors?

Sushilkumar Devidasrao Ramdasi @Sushilkumar_Ramdasi

01 January 2019 1 691 Report

Hi,

Please guide me on area classification and negative pressure specifications for cGMP laboratory for the production of clinical grade viral vectors.

Also, please help me with relevant regulatory guidelines.

Thanking you in anticipation.

Paolo Sanesi

To start please read this official guideline: https://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf?ua=1

Let me know

Badges
Science topic

Similar topics
Data Mining
Classification

More Sushilkumar Devidasrao Ramdasi's questions See All

How to culture 3D spheres of MSCs?

I am working on 3D culture of MSCs i.e. shpere formation and culture. I would like to know how to culture these 3D MSC spheres in culture vessel other than spinner flasks. I would like to maintain...

10 November 2017 6,170 3 View

What is risk based developmental quality assurance in biotech industry?

can I get information on risk assessment in developmental quality assurance in biotech industry. Please add references and tools for risk assessment.

07 August 2016 7,447 0 View

How to identify and characterize A549 lung cancer cell line

Please guide me on how to identify and characterize A549 lung cancer cell line by molecular technique

11 December 2013 667 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

25 July 2024 8,108 0 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View