We need the details of RLFP or ARISA methods which can be used to research genes responsibles of proteins production.
Dear all, I'm wondering about a possible substitution of a G>A base or a deletion of the G base in view of the chromatograms I've analyzed. I would like to point out that I have observed the...
21 November 2023 8,904 2 View
Dear all, as part of my Master thesis, I am conducting a two-way repeated measures ANOVA on data from a social evaluation paradigm. The purpose for running this analysis is to investigate whether...
19 October 2023 3,746 1 View
Dear Sir/Madam, I am looking for a deletion or insertion anomaly in the CALR gene. After sequencing the same sample (Forward/Reverse), here's what I get. From the chromatograms we deduced that...
09 August 2023 4,907 4 View
I recently noticed that the BSC-DREAM8b-Dust model can no longer be accessed. http://www.bsc.es/ESS/bsc-dust-daily-forecast Do you have any idea if it can be accessed from another database or if...
11 March 2023 2,604 3 View
I want to culture and store fungal spores of aspergillus using glycerol and need help with the procedure from anyone using it or have used it.
14 September 2022 9,087 3 View
Hello scientists. I am seeking for natural compounds with anticancer activities. Actually I am interested to hepatocellular carcinoma (HCC). Unfortunately I am facing some issues to induce HCC...
09 June 2022 5,430 0 View
Hello my Dear, I have a question about CALR chromatogram analysis. I sequenced and aligned the amplified sequences with the reference sequences but I'm having trouble identifying the anomalies...
13 April 2022 756 8 View
Hello, recently we have been working with the cyanobacterium Arthrospira platensis (Spirulina) and noticed, in shake flask culture cultured on an orbital shaker at 100 rpm, spherical cells and no...
01 February 2022 6,501 5 View
Greetings, we are currently working on the cultivation of the microalgae Dunaliella tertiolecta. We try to count the cells in a Thoma cell counting chamber under the microscrope. Problem is, the...
10 August 2021 7,640 4 View
Currently we are working on the cultivation of Aspergillus oryzae in shake flask liquid culture. To inoculate the cultures, a certain concentration of spores in the inoculum is wanted (Spore count...
11 March 2021 5,111 8 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View