What methods of isolating DNA of gram-negative bacteria in/from insects that can be done?

More Harold William Bonar Manihuruk's questions See All

How can microbiology skills and training be improved to reduce the misidentification of commensals and contaminants as pathogens?

This question is related to the review article titled "Tenets of Specimen Management in Diagnostic Microbiology" by Rajeshwar Reddy Kasarla and Laxmi Pathak. The article states that optimal...

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Are telescope lenses or mirrors---or the lenses of our eyes---necessary for the maximal detection of individual photons from distant galaxies?

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08 May 2023 1,464 10 View

Is there an optimal placement distance (from the eye lids), for vertical EOG electrodes?

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06 October 2022 397 1 View

Free DEA solver or add-in?

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01 June 2022 3,552 1 View

I want to test the difference in knowledge, skills and attitude of resp when grouped into age gender, civil status. what stat should I use?

23 August 2021 2,402 3 View

Can I ask help about Related research with regards to Solid Waste Management?

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20 March 2021 1,295 3 View

Band Structure Calculation from check (castep_bin) file CASTEP?

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20 October 2020 4,427 0 View

How to implement optical CDMA using optisystem and matlab all together?

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19 March 2020 1,750 6 View

Using satellite images where I intend to get image data for supervised classification, how do i differentiate households from commercial bldgs?

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20 May 2019 2,438 3 View

Are there any available doctoral level supervising Professors within the field of the Circular Economy here?

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19 April 2019 1,087 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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07 August 2024 6,034 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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Abhijeet Singh

What is your purpose to isolate DNA or aim of project?

What insect?

>> Would be very much helpful if you can take some time find find relevant literature and also understand some fundamentals.

Robert Adolf Brinzer

You probably want to lyse the host cells with some kind of eukaryote specific toxin before enriching for your bacteria with centrifugation or filtration before doing DNA extraction.