I am to collect mucosal scrappings, not faeces, from domestic birds. How do I quantify the number of oocysts present? Is it possible for me to use a floatation technique to concentrate the oocysts and then count? Or is there another scientific method I can use in counting directly from wet preparations?
Dear Oluwayomi Adeyemi
Pls. find the attached files
http://avmajournals.avma.org/doi/abs/10.2460/javma.232.5.733
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937059/
http://www.sciencedirect.com/science/article/pii/0014489470900731
http://link.springer.com/article/10.1007/s00580-014-1986-7
http://scialert.net/fulltext/?doi=ajpsaj.2012.79.88&org=10
http://www.m.elewa.org/JAPS/2013/17.2/1.pdf
http://www.thepoultrysite.com/publications/6/diseases-of-poultry/206/coccidiosis/
http://www.sciencedirect.com/science/article/pii/S0304401708000927
http://www.sciencedirect.com/science/article/pii/S0749072009000231
http://www.sciencedirect.com/science/article/pii/S0304401701006100
Hoping this will be helpful
Regards
Prof. Houda Kawas
You can use the McMaster Technique for quantification of Eimeria oocysts directly from wet preparations. As far as I know, you should never concentrate the sample by floatation or any other technique before counting.
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