What materials and equipment are needed to cover the seeds of rapeseed?

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How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How post-hoc test should I perform for two way repeated measures ANOVA for 3 groups?

Hello everyone, I have 3 groups and 4 measurements so, I performed two-way repeated measures ANOVA. How can I detect the difference (which groups and which measurements) when there is significant...

27 October 2020 4,503 3 View

Is there any correlation between theory of mind and imagination?

Is there any evidence to address the correlation between theory of mind and imagination? The idea comes from daily experiences demonstrating a possible relation between the power of imagination...

10 October 2020 7,369 9 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Do weak contribution equations within a module use the module's boundary condition in COMSOL?

I am trying to solve a partial wave equation for scattered quasi-TE waves. I found the COMSOL built-in Helmholtz equation module very close to my problem. The bottleneck was that, I couldn't...

03 June 2020 8,937 1 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

25 May 2020 7,276 9 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

How should I compare pre test and post test of three groups?

Hi everyone, I have 2 treatment groups and 1 control group. I measured some physical parameters as a pre-test and post-test. Should I compare 3 groups pre-test and post-test separately by using...

23 March 2020 8,599 8 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View