Do you know a universal statement, which is currently actual ?

In my opinion "each medal has both sides"

31 December 2018 7,824 2 View

What is in your opinion the best approach to identify diabetic retinopathy as soon as possible ?

Screening, teleophthalmology, artificial intelligence, etc.

09 October 2018 2,645 0 View

Could artificial intelligence replace the Ophthalmologist in diagnosii of Diabetic Retinopathy ?

Currently available findings indicate success of AI in ophthalmology, specifically in DR screening process. Your opinion will be highly appreciated

06 July 2018 8,718 5 View

How achievements in RG such as new citation, recommendations for answer, continued reads of papers correlate with decrease of RG score ?

Who has an experience when during one week despite mentioned achievements such as new citation, reaching 200 reads of paper, 10 recommendations of the answer, continued reads of papers reaching...

05 June 2018 9,207 11 View

How to regain the lost public trust in healthcare?

How to regain the lost public trust in healthcare ?

05 June 2018 6,676 3 View

Analytic thinking: Is it Mandatory in Medicine ?

Analytic thinking: Is it Mandatory in Medicine ?

04 May 2018 8,946 3 View

How to evidence clever person from unclever one ?

A smart person is eager to learn and unclever -eager to teach Anton Chekhov

04 May 2018 5,577 2 View

Who is the best : Artificial Intellegence or Human ?

Currently AI is more and more involved into medical diagnosis, specifically for diabetic retinopathy, etc. Your opinion will be highly appreciated

04 May 2018 5,756 5 View

Do you have any suggestions on the " colour" of envy ?

Some people divide an envy on white one supposing it is not negative sense in contrast to " black" one

01 February 2018 5,398 17 View

Could environmental factors be risk factors for diabetes ?

The latest research have shown increased 2- and 3-fold diabetes related with plastics, phthalates, hear spray, flooring, pesticides.

11 December 2017 4,980 12 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View