Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How I can to estimate the surface area of a given amount of graphene?

I need to know the amount of graphene, to cover a surface.

30 May 2020 6,297 4 View

Why neuromyths (misconceptions of brain and cognitive function) are still present in education?

We seek reflections of professionals and academics of education, for the installation of neuro myths in education in Latin America

26 December 2019 2,254 3 View

Can you include a focus group with only one participant in your data analysis?

We are analyzing data from a qualitative research study in which we conducted focus groups. However, for one focus group, only one person showed up. We went ahead and did the focus group, but can...

04 November 2019 4,335 11 View

How long after my last allergen challenge can I measure Mouse Mast cell protease-1 (mMCP-1)?

Hi: I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues...

15 October 2019 9,291 2 View

What dilution should I use for my mice lung homogenates to perform a BCA total protein?

Hi community: I was wondering if you could give me more tips on the following. I am looking at cytokines levels of lungs exposed to an allergen. Specifically, I am looking at intracellular...

25 September 2019 6,686 7 View

How to add a Journal to the available ones when adding and article to ResearchGate?

11 December 2018 7,043 0 View

How to eliminate erratic peaks in NanoDSC, TA Instruments??

Anyone with a Differential Scanning Calimeter (NanoDSC, TA, Instruments) has had erratic peaks during the scans?... We have use all type of cleaning protocols and eliminated bubbles, but the...

03 April 2018 5,864 3 View

Do you know any community-level dataset with at least 20 years since the first survey?

I'm looking for community-level datasets older than 20 years, at the landscape spatial-scale (i.e. multiple survey points in space), for any taxa or region. If you know any dataset with these...

26 December 2017 4,467 1 View

How can i improve standard curves values in RT q-PCR miRNA assay?

We are using TaqMan Advanced kit, the efficiency in most of our standard curves is 150%, with low slopes (-2,6) but high R2 (>0.99), how can I improve results?Has changing the dilutions...

21 July 2016 1,948 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View

How to calculate stress at the cross section of spherical particles within a matrix?

Hi all, I am currently working on some background calculations before I start a new project related to metal matrix particulate composites. I am trying to find a way to calculate the stress at...

19 February 2021 8,503 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View