l am trying to HPLC purify a peptide which is rich in polyglutamic acid. Our common running solvents are water and acetonitrile with 0.1% TFA. Is it possible to get rid off TFA after columns and everything else is well conditions after so many years? What kind of running solvents would someone suggest in order to purify a rich glutamic acid peptide? It kind of seems to me it is not so straight forward to change the composition of the mobile phase after so many years.
Damien Habert
get rid TFA : run =
10 min 5% acetonitrile without TFA
80 min 80% acetonitrile
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Fernando Reyes
TFA can be easily eliminated using a rotavaporator. Its boiling point is 72.4 C. Unless your peptide is unstable under acidic conditions you do not need to make any change in your mobile phase.
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