If we measure large (up to micrometer) Janus particles with differently charged surfaces, what would be the resulting Z-potential? Two separate peaks representing each surface or mean value between them? Thank you!
Could you please mention the purpose of this study? I have a long experience of estimating ZP of manganese oxide particles.
Measurement of the zeta potential of colloidal particles using electrophoretic methods allows one to determine only the effective average potential of a particle, which can sharply differ from local values. Local values may not be uniform. Any method of experimental research contributes its interaction with Janus. Numerical modeling is also not an option, as it chooses its potentials.
Electrophoretic light scattering should give you an idea of the effective "net" charge of a particle. If you have one class of particles that have an effective zeta of 10mV and another class of particles with an effective zeta of 40mV then you may be able to see two peaks in the zeta potential distribution. However, this requires that both components contribute to the scattering in a detectable ratio (i.e. not one overpowering the other).
If there are different charges on one particle then all that matters is how the total particle moves in the applied electric field. If they would separate, you may see two peaks in zeta distribution, but otherwise not.
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