What is the volume of LPS should be added in each well to induce changes in RAW 264.7 cells using 24 well plates?

02 February 2020 1 7K Report

Im currently working with RAW cells. Previously, i seeded the cells in 96 well plates, after 24hrs i add LPS in each well (100ul each well, 5microgram/mL). So i want to know, what is the volume that i should add in each well if im using the 24 wells plate to induce the same changes as in 96 wells plate.

Elena Carelli

Hi ,

I suggest you to add 500 ul of your solution in which you have LPS.

Bye!

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