Using Image J for histological analysis the area measured has its unit as percentage (%). What about the mean gray value or gray value?
Is it that the NIH approved software for histological analysis has no unit for gray value?
I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]
04 January 2021 5,528 2 View
Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...
18 November 2020 6,412 7 View
I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...
22 March 2020 4,860 7 View
I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...
24 February 2020 8,142 4 View
If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...
03 December 2019 273 4 View
Hello everyone, at the moment I'm looking for a topic for my bachelor thesis. I was thinking about Blockchain and Smart Contracts for cyber-physical Systems, in order to use it for Smart Factory...
23 October 2019 3,694 5 View
Hi, I want to carry out western blotting for a 233 kDa protein. I was told to use a tris-acetate gel due to the size of the protein. However, I was wandering would I be able to detect the...
25 March 2019 6,308 4 View
Dear Community, I recently switched from ANSYS Mechanical to COMSOL Multiphysics. So far I was able to transfer my models to the new platform but I yet miss one feature of ANSYS in COMSOL. I hope...
29 January 2019 4,937 3 View
I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...
13 November 2018 6,049 3 View
I want to set different thresholds for calculating the NDVI images using Sentinel-2 for the purposes of fishing out river sediments in the Dry and Wet seasons. I am using the Semi-automatic...
05 November 2018 694 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View