24 November 2020 1 2K Report

I have a methanol-water mixture and I want to know how temperature changes pressure with varying methanol-water percentage (v/v)?

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I am carrying out MTT Assay using HeLa cells but the absorbance values are really less, can anyone please help?

I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...

12 June 2024 1,460 3 View

How can we do an RMSD validation for a protein which has 2 native ligand binding sites?

I have done an RMSD validation for such protein by docking the two ligands one by one, but each docking results in large RMSD value compared to the reference.

22 January 2024 2,054 5 View

How can I get a prominent band of Phospho-mTOR (289 KD) in western blot ?

Hello everyone, I am performing western blot (SDS-PAGE) for the phospho-mTOR protein, molecular weight 289 KD. I am using 6% resolving gel for it. I kept the transferring time around 4 to 6 hours...

18 January 2024 1,061 1 View

On aqueous extraction of oil what is the white milky layer that is obtained as supernatant?

I am trying to extract lipids using water as solvent. After extraction followed by centrifugation, I did not obtain any oil layer. Instead the supernatant was milky white, which I think might be...

30 October 2023 9,151 1 View

How is steam jet ejector added in Aspen Plus?

Steam jet ejectors are not present in the built in models of Aspen Plus. How to add a steam jet ejector in Aspen Plus. Can it be done by the combination of two models or are there any other...

18 October 2023 5,200 1 View

In multi-effect distillation for seawater desalination, the effects are operated in subatomic pressure. How is this pressure maintained?

Usually MED is accompanied by a steam ejector or compressor. How is the subatomic pressure maintained inside the effects?

17 October 2023 4,447 3 View

If there is confinement of phonon in a system, how it would affect the phonon lifetime?

Whether the lifetime will increase or decrease w.r.t to a system which has no confinement? Furthermore, let me know, how I can use PCM model and extract the Raman profile for a particular range...

12 October 2023 6,795 1 View

How to calculate electrochemical active surface area from Cyclic voltammetry or Nyquist plot?

Electrochemical active surface area (ECSA) from CV or EIS

16 September 2023 9,729 4 View

What could be the reason of multiple surges (bumps) in the IRF and decay profile of the TRPL data for weak fluorescent material ?

I took an IRF using deionized water in the TCSPC system, in which the decay profile showed multiple peaks instead of smooth exponential decay.

10 July 2023 5,232 4 View

Can anyone share the CIF File of NiFe LDH?

I want to perform some DFT study for which I require the CIF file. I searched COD and materials project, but it's not there.

24 June 2023 8,116 2 View

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