Hi, i am a postgrad student who are currently doing malaria research and i will do Beta hematin inhibition assay. i would like to ask two questions.
here's the formula i used to calculate the % of inhibition.
f = (Acontrol -Asample)/ (Acontrol –Amin)
Acontrol is the absorbance of the heme with the absence of parasite lysate or lipid extract or an antimalarial compounds at 405nm while Asample represents the absorbance of the heme in the presence of both parasite lysate or lipid extract and crude extracts or major compounds. Amin is the absorbance of the heme with parasite lysate or lipid extract in the absence of an antimalarial compounds at 405nm. Percentage of inhibition of β-hematin by plant extracts will be calculated by the following equations
% of inhibition = (1-f) x 100
Thank You in advance.
Always include a control without parasite (extract only) and normalize.
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