What is the storage condition for IPTG solution? What is its toxicity level for BL21 cells during induction?

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Panduranga Rao

IPTG is not light sensitive (its colleague X gal is).

It is not toxic to E coli at concentrations below 1mM (but the protein induced by it may )

Gaurav Kumar

Thank you both for the clarifications.

Aydın Yeşilyurt

What if we forget it at room temperature overnight ?

Surojit Sural

Aydın Yeşilyurt, it should be fine. The following information for IPTG is provided by Sigma-Aldrich:

The IPTG solutions may be aliquoted and stored at -20°C. IPTG solutions can be stored at room temperature for up to one month. Maximum IPTG product life can be achieved by avoiding repeated thaw/freezing.