What is the standard protocol for the microbial extraction for FAME identification and analysis?

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Is it possible to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells?

I have been trying to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells using the MOMP and Plasmid primers respectively without success. So far only...

01 February 2018 4,675 2 View

How do I design degenerated primers for RT-PCR flower and fruit gene expression in pearl millet using arabdiopsis using ABC model?

I am trying to quantify and compare genes expression for germination, flowering and fruiting in pearl millet and napier grass using Arabdiopsis thaliana using ABC model.

11 December 2014 7,053 3 View

What diploid DNA plant species can I use as control in DNA ploidy analysis of pearl millet and napier grass using flow cytometry technique?

I would like to analyze DNA ploidy level in pearl millet and napier grass using flow cytometry method. I would require a known ploidy DNA plant as control. Please, kindly help out if you know for...

10 November 2014 976 4 View

Can anybody tell me how to design primers for quantitative Real Time -PCR?

to quantify the functional DNA per set of genome in Pennisetum glaucum (Pearl Millet) and pennisetum purpureum (Napier grass)

02 March 2014 7,872 14 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View