Grow the bugs until growth reaches logarithmic phase. Centrifuge, collect the pellet by resuspending it slowly in 50% glycerol. Homogenize gently, then aliquot 50-500ul in 2ml nalgene vials (screw cap) and snap freeze in liq nitrogen. Transfer to -80C asap.
Grow the bugs until growth reaches logarithmic phase. Centrifuge, collect the pellet by resuspending it slowly in 50% glycerol. Homogenize gently, then aliquot 50-500ul in 2ml nalgene vials (screw cap) and snap freeze in liq nitrogen. Transfer to -80C asap.
Well ,this is rather good way to preserve, do you tell me that how we can preserve the biomass directly from the colonies. instead of growing in broth.