Are you trying to make your own system?  I would purchase pre-made real-time PCR mix from company.  It would make your life much easier, and given the nature of the assay, the results you get would be more consistent.  But, if you must know: it contains high efficiency taq polymerase, MgCL2, and other buffer components specific for the enzyme.  It also contains some sort of fluorescent dye for ds DNA detection.  Cannot tell you what the concentrations of those are.  But, the basic components are those.

Water is in every buffer, too ;)

qPCR buffers are in principle not different to normal PCR buffers, with the exception that they contain some biochemical ("probe") to generate a fluorescent signal that is proportional to the amount of PCR product.

qPCR buffers usually have highe Mg++ concentrations (4-6 mM).

The primers will be added to the buffer, for sure. For qPCR, the primers should be designed to to amplify a rather short product (80-100 bp) with optimum efficiency and without producing unspecific products.

There are tons of qPCR protocols freely available in the internet. Have you searched?

There are also many many books describing all that in detail. What books have you read?

http://lmgtfy.com/?q=qPCR+protocols

Hello Timothy,

I have nothing to add to what Jochen said. You can use the same reagents for qPCR that you use for endpoint PCR. All you need is some fluorescent dye like SybrGreen.

There are a many manufacturers who offer ready-to-go mixes for probes and SybrGreen assays for very reasonable prizes. So don´t bother spending a lot of time on setting up your own mixes.