What is the stability and viability of CD133+/34- cells? Is it possible to analyze (with relevant results) even the day after bone marrow collection?

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Gopi Krishna Kolluru

I presume that you are using flow cytometry and mouse model. Cells isolated from the bone marrow better be analyzed on the very same day otherwise it effects the viability and also clumping issues.

Peter Kubisz

Dear Gopi,we are using flow cytometry only in humans.With cytometry we use also /for viability/trypane blue staining.Viability is 75 to 95 per cent (after 16 h)and our procedure is successful,for.ex.see: Therapeutic angiogenesis for the salvage od ischemic limb,CLAHT,September 1-3,2011,Montevideo,Uruguay