What is the impact of Washback in teaching a language or any other subject?

The importance of washback in teaching and learning the language through assessment

15 November 2020 3,559 4 View

How can we use 'assemblage theory' by multi-disciplines for designing a better children environment?

A research group is conducting a study across several cultures to investigate insights about issues in the environment of school-age children (6-12). The purpose is to explore practitioners'...

05 August 2020 1,967 5 View

Can you help in the two-minute survey about nightlife atmospheres in historical places?

19 July 2020 9,584 15 View

Load deflection curve in abaqus?

Ihahve modeled RC beam in abaqus, but the load decrease extremely after the maximum is reached, what could be the problem?

03 May 2020 2,566 8 View

I want to know the relationship of seed oil contents (Gadoleic acid, erucic acid, thioglycoside) with seed germination and drought stress in plants?

hello everyone, I want to know the relationship of seed oil contents (Gadoleic acid, erucic acid, thioglycoside) with seed germination and drought stress in plants?

26 April 2020 7,026 2 View

What is the difference between connector type axial and friction in abaqus to define a bond between concrete and steel?

what is the best way to simulate the bond between steel and concrete if i will use connector, what is the difference between connector type axial and friction how i can define shear stress...

25 April 2020 5,880 3 View

Can I use anhydrous ammonium phosphate in oil and grease extraction?

hi, can I use anhydrous ammonium phosphate instead of anhydrous sodium sulfate in oil and grease extraction?

23 March 2020 9,987 3 View

I need some genes related to drought stress and oil contents in germination stage in Brassica napus?

Hello everyone, I'm working in Brassica napus under drought stress and oil contents in germination stage and I want to find relation between both of them so can any one tell me some genes related...

24 November 2019 5,807 5 View

What are the qualities of an excellent doctoral student? Can one detect his qualities before hiring him? How?

Very important question to avoid surprises...

01 July 2019 1,117 17 View

1- OHS in the industry 4.0 era: A cause for major concern?

In the midst of this new and accelerating industrial trend, are we giving due consideration to changes in OHS imperatives?

28 May 2019 8,723 7 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View