16 February 2018 3 7K Report

What is the spiking protocol we have to follow it to detect LOD / LLOQ) in rare event flow cytometric assays such as ( CD34 enumeration ) ?!

More Abeer Badri's questions See All
Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

How to submit an article?

steps scopes

07 April 2024 1,171 1 View

Field notes: Where do you write in mixed research in any chapter?

Field notes: Where do you write in mixed research in any chapter? In the procedures chapter or the results chapter? "In doctoral thesis"

31 March 2024 5,472 3 View

How do we use language to confront extremism and terrorism?

How do we study the expressions that contribute to the spread of extremism in society and how do we work to confront them through expressions that encourage peaceful coexistence?

25 January 2024 6,912 1 View

Hello, I have two Independent variable, One dependent variable, one moderator, and one mediator. So now I could not find suitable conceptual model?

Can anyone of you please share the right conceptual model for this variables? I have two Independent variable, One dependent variable, one moderator, and one mediator. So now I could not find...

24 December 2023 398 0 View

What is the difference between TGA and DTG, I need interpretation for these graphs attached?

I need your help as soon as possible, thank you

10 November 2023 7,998 0 View

Is it possible to increase dislocation density in an elastically loaded metal by heating it to a sufficient temperature?

If we take a metal which is loaded elastically (near the yield point but still elastic) and heat it to a sufficient temperature, such that its yield strength drops, some of the elastic strain will...

24 September 2023 2,885 2 View

Sorry, I posted this question by mistake?

thank you

24 September 2023 1,348 0 View

Strain selection for plant proteins fermentation?

Is a lactic acid bacteria strain sourced from plant sources is more suitable for fermentation of plant proteins compared with a dairy sourced strain?

18 June 2023 2,805 1 View

Which lactic acid bacteria has the highest/strongest proteolytic activity?

I am working on a project to use bacterial fermentation of proteins from plant-based resources. I was wondering if anyone comment on which lactic acid bacteria i.e. Lactobacillus helveticus or L....

18 June 2023 690 1 View

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View

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