What is the specific process of standard PCR?

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Can the size of an empty micelle be larger than the size of the micelle in which the ingredient is encapsulated?

The particle size distribution of the micelles was measured using a particle size analyzer. The dv 50 value of the empty micelles was about 2 um larger than the dv 50 of the encapsulated micelles....

19 July 2024 803 4 View

Any advice on analyzing two-factor gene expression study?

Hello Research Community, I am currently working on a gene expression study involving two factors: treatment (control vs. treatment) and time points. The results from a two-way ANOVA using GLM...

06 June 2024 2,310 2 View

What are the roles of indirect and direct band gaps in nonlinear absorption?

What are the roles of indirect and direct band gaps in nonlinear absorption? If it's convenient, please provide me with some articles. Thank you

21 January 2024 8,377 1 View

Hek293 T cell culture - strange cell morphology after dissociation?

I borrowed a bottle of HEK293T cell culture from someone else, but after one passage, the cell morphology became very strange and not round at all. May I ask why? We used T25 culture flasks and...

05 January 2024 5,910 4 View

Ask the Multilevel regression independent variables?

Whether one independent variable in multilevel regression can be the other two independent variables, such as type, token, and type/token ratio

27 December 2023 8,834 4 View

The oscillation range of the photovoltaic frequency in this picture is very small, about 0.02Hz, can it be used in industry?

The oscillation range of photovoltaic frequency in this picture is very small, about 0.02Hz, can it be applied to microgrids? Is this a low frequency oscillation?

16 October 2023 2,823 5 View

Should the induction motors be directly connected to the grid?

When considering dynamic loads (induction motor loads) in an isolated microgrid, should the induction motors be directly connected to the grid, or should they be connected to the grid through an...

09 October 2023 8,895 3 View

How does eye fatigue evolve over time with long office hours?

With the increase of office hours, the human eye will produce visual fatigue, but with the increase of time and gradually deepen the visual fatigue, how is the change process？Working on different...

14 May 2023 9,706 1 View

CMIP6-based precipitation and temperature data processing?

Now I want to access the two future simulations of temperature and precipitation in the CMIP6 dataset (in ssp126, ssp245, ssp370, ssp585 senario). It is only necessary to obtain the data for the...

09 May 2023 9,756 1 View

How about focusing the laser to less than 1um without losing most of its energy?

1. I am currently using a 532nm laser and NA = 0.6 objective lens for laser focusing, (the spot size of the laser outlet is 2mm, then expand the beam 4 times, and finally through several mirrors...

25 April 2023 7,181 11 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View