Dear Roaa, starch is water soluble than other solvent, you can use some solvent like chloroform and propane but not strong. i suggest you to dissolve starch by water. starch dissolving is by digestion and water is very good solvent ok
@ Roaa, Hot water is a very good solvent for Starch. Generally starch dissolve in polar solvent. ZnCl2 aqueous solution also reported as good solvent of starch.
The purpose of your research is not completely clear, so I restrict my contribution to a few suggestions. You may select the approach most suitable to your research.
1. Most solvents only have a limited effect on granular starch at common conditions: their action is to swell the granules with molecular solubilization always < 100 %. Under the microscope (preferably with phase contrast optics) swollen granular structures are still visible. Examples: water (60-100 C), 1M aqueous NaOH (room temperature), 100 % DMSO, 90/10 DMSO/water (room temperature).
2. True molecular starch solutions can be obtained by heating in water (140-160 C) or in 90 or 100 % DMSO (up to 100 C), in combination with mechanical agitation (homogenization, jet-cooking). Suspensions in 1M NaOH must not be heated but only agitated. However, some molecular degradation of the starch will occur. Development of yellow or brown color in your solution points at severe molecular degradation.
3. One protocol to obtain molecular starch solutions without degradation is to dissolve the starch in 90 % DMSO, precipitate with ethanol and redissolve it in boiling water (Yoo et al., suitable for high dilutions only, see attached file).
4. If you really want water-free solvent systems the use of ionic liquids and deep eutectic solvents has been described (BIswas et al, see attached file). But these are not 'bio-solvents'.