What is the simplest method for determining the activity of dioxygenase enzymes in bacteria?

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Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

How to simulate a metalens with backward propagating source using a Lumerical FDTD?

Dear Sir/Madam, I am currently working on a beam shaping problem and have referenced the Metalens example provided on the Ansys Lumerical website. In that example, a forward propagating plane wave...

14 June 2024 4,333 0 View

Do you know a partner, friend, colleague, or someone else who was a Canadian gay (male) nurse who cared for PLWH during the HIV/AIDS pandemic?

My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...

10 June 2024 6,253 0 View

Protocol for Coating Plates with Collagen?

Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.

22 April 2024 6,835 1 View

How do I determine and what radical is this in this EPR graph (DMPO)?

So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!

06 April 2024 7,084 3 View

Is it required to send interview transcriptions for publishing qualitative research in a journal as part of data availability?

As someone engaging in ethnographic research, are we expected to disclose transcribed data to the journal where we would like publish an article?

03 March 2024 3,653 3 View

Which Promotor Sites can I use for In Vitro Transcription?

Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors...

26 February 2024 1,852 1 View

How should one teach ETHICS to the new generation of college students in 2024?

More and more, teaching ETHICS has become an important part of the college curriculum, but teaching it has not always been easy or up to part with the generational changes in the student...

02 January 2024 7,862 6 View

Buffering pH of DNA Binding Solutions for Silica Columns?

I am trying to prepare some custom DNA binding solutions for purifying DNA with silica columns. I have prepared one with 4 M guanidine thiocyanate, 30% isopropanol, and 10 mM Phosphate buffer, pH...

03 December 2023 2,197 0 View

How do we design a three level dimensions of organizational ambidexterity?

I am currently exploring on the concept of "Organizational Ambidexterity". I would love you give me an insight on the way to design its dimensions in a three level scale, where it will depict its...

27 October 2023 3,058 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View