I want to extract endogenous auxin and cytokinin from my rice callus. Can anybody give me suggestions what kind of method should I use? I only have a few amount of callus so I need effective method. thankyou
use HPLC following solvent extraction. make the pH of extract acidic-2-3 for the extraction of auxins, gibbberellins and ABA, then change the pH of the extractto basic-pH 8.5, to extract cytokinins,please see my published work on Rsearch Gate,