Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to plot non-linear kinetics model for column sorption study?

Do I have to solver-add inn for its calculation?

10 December 2020 5,209 7 View

Why many biochar producers find it difficult to sustain in different parts of the world?

Biochar (solid product with more than 60% carbon as per IBI) has been extensively studied for its production, characterization, and applications. In spite of offering numerous benefits in diverse...

23 April 2020 9,534 1 View

Cloning a 4.3kb insert into 6kb vector?

I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...

24 November 2019 9,429 2 View

Hii everyone, my spss paired t test value is coming different when i calcualte manually. i have checked many times. which is correct

my sample size is 43. when i am applying in spss its coming 13.5 and manually 0.88, what should i do

31 May 2017 2,409 5 View

Which metals ions form complex with sodium potassium tartrate?

Can +3 cations form tartrate complexes?

25 October 2015 3,094 3 View

Are there any model specification tests with panel data?

Hi I am working on count panel data. Is there any test i can use to test model specification. Linktest and ovtest are not applicable.

23 May 2015 9,295 2 View

Where could I get the cost and schedule data for various framework systems such as Peri, Mivan, and Doka?

Form work optimization in construction goes a long way in bringing efficiency into the construction process.

06 April 2014 4,992 1 View

What is the best way to precipitate silica particle from TEOS (tetra ethyl orthosilicate)?

i want to use TEOS as silica precursor in my catalyst preparation. My catalyst is cobalt based on silica.

11 December 2013 4,186 9 View

Agnuside and P-hydroxy benzoic acid

Agnuside and P-hydroxy benzoic acid are both phytochemical of Vitex negundo but the polarity of both the compounds is unknown. Which one is more polar?

21 May 2012 4,668 6 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Helix antenna coupling problem?

Dealing with a helix antenna, made with flexible material and not rigid like using a PCB board or wire. At the beginning of the helix is too close to the ground plane, causing coupling. How can I...

21 February 2021 4,453 3 View

Which method need to be used to predict crack propagation?

Need to predict the crack propagation phenomenon in a CI component. Suggestions are needed regarding the the methods ( XFEM, J integral, etc.,) which will predict the crack propagation behavior...

06 February 2021 1,936 4 View

How accurate is the shear modulus measured using PROCEQ DPC transducer of 40kHZ frequency on 100mm concrete cube specimen?

It is required to evaluate shear modulus of concrete cube specimen of 100mm size. Since the diameter of DPC transducer is around 84mm, I would like to know whether it can give reliable shear wave...

02 February 2021 1,052 3 View

How to solve 1-D Wave equation?

Good day, In standard PDE literature, there are a lot of solution approaches for the 1-D wave equation initial-boundary value problem. One way is with separation of variables. Below I describe...

25 January 2021 4,876 3 View

What do you mean by 'excitation' of a plasma wave? What is the physical significance of this 'excitation'? Why is it necessary?

I have read numerous research papers on various mechanisms of excitation of surface plasma wave. But why do we need to excite a wave? Is it necessary? Can't the wave propagate without being excited?

25 January 2021 3,790 3 View

I am working with crack propagation along with delamination using ABAQUS. Wether crack will start from a delaminated portion (cohesive crack) by XFEM?

I am modelling a SENB Beam with an interface. The interface is modelled with cohesive elements to permit delamination. The carck in the continumm is taken care by the XFEM. My doubt is the crack...

19 January 2021 4,827 1 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

What type of spectral radiation has greater penetration on materials, UV or IR? What book or source do you recommend for information on this?

I need information concerning the penetration and reflection capacity of ultraviolet and infrared radiation wavelengths on different most common materials. Edit. Can somebody recommend a book to...

16 January 2021 8,051 6 View