Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

Do those who are uninsured in the USA receive Covid-19 inpatient treatment?

I am drafting a paper on the number of hospital beds in the different States of the US and find that half of US States have bed provision equivalent to the less developed countries. This leads to...

16 April 2020 2,775 10 View

Is my initial AP FRET set up suitable?

Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...

09 March 2020 2,349 2 View

How to compare two curves using stats?

I am trying to determine if an equation is suitable to model Pressure drop vs fluid velocity. I have experimentally measured pressure drops for a variety of velocities, forming a curve, and I...

06 March 2020 9,517 8 View

What statistic do I use on non-parametric data where I have a response variable and two interacting categorical explanatory variables?

I was wondering whether anyone had any suggestions as to what statistic to use (I use R Studio). I have some muscle cell circumference measurements (response) and I have two explanatory variable...

27 January 2020 5,835 3 View

What are good Hypothalamus Nuclei Markers?

Does anyone know of any good hypothalamic nuclei markers that can be used for immunohistochemistry purposes?

17 June 2019 4,018 3 View

Ways to validate qualitative research?

Using: Husserlian phenomenological qualitative research, eidetic bracketing, semi-structured interviews & Colaizzi data analysis. What are additional ways to validate research given I will be...

05 June 2019 537 6 View

What is the model of computation for this device?

In our research we have been given a device that may be capable of computation. To determine if this is or is not the case we looked for what could be the logical primitive in the physical...

02 March 2019 1,907 1 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 2,121 4 View

How do I calculate output of solar panels for a given area?

Hi, I'm a little unsure if I have the correct formula. I'm looking to calculate the potential maximum output for Photovoltaic material when I know the surface area. i.e. peak= Wm^2 x total area...

02 March 2019 9,949 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View