What might be the lowest concentration to get the minimum signal to noise ratio in an NMR experiment?
Dear Jinal Pothupitiya,
There is no simple, one-sentence answer to your question. The amount/concentration of sample depends on many factors; the type of NMR experiment (e.g. simple 1D 1H and 13C; other nuclei (e.g., 15N, etc.) various 2D (e.g., 1H-1H NOESY/ROESY, 1H-1H COSY, 1H-1H TOCSY, 1H-13C HSQC, 1H-13C HMBC, etc.); the equipment (spectrometer, probes,etc.) available, etc. Modern, high-field spectrometers equipped with cryoprobes
(cryogenically-cooled probes) have significantly decreased the sample amount requirements.
As a rough guide, please see the attached publications free on the Internet.
Depending on your equipment, the manufacturer (e.g. Bruker) can give you specific information on S/N, etc.
Also, your question (or similar ones) have been posted in the past on ResearchGate by Fuad Al-Rimawi, and by Mohammad Nazrul Islam Bhuiyan.
I hope the attached information is of help to you. Good luck in your research! AF
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