What is the role of methanol in transfer buffer?

06 June 2014 1 3K Report

Western blot.

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Science method

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Can anyone help with mf gel PAGE?

While doing gel PAGE, my molecular weight marker did not stack. It started resolving in stacking gel itself. I have checked pH of all buffers, they wr 5ne.

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

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What's the concentration recommend for recombinant protein use in western blotting?

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Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

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What is the best way to manage Western blots?

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How to manage western blot for proteomics data validation?

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Diltuions for a Western blot?

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Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

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