While doing gel PAGE, my molecular weight marker did not stack. It started resolving in stacking gel itself. I have checked pH of all buffers, they wr 5ne.
Hi Purva,
If you are making the separation gels yourself, have you checked the stock % of acrylamide you are using? Sometimes the stock solutions you buy commercially are 40% and not 30%. Perhaps this could be issue, with the stacking gel having a % too high through a miscalculation?
Again if you are making the gels yourself, you are using the correct buffer chemistry in the gels themselves? That is either Bis-Tris or Tris Glycine? Perhaps your running buffer is not compatible with your gel buffer?
Glad to hear you have trouble shooted to fix the problem. Good luck with the rest of your experiments/
i said it was fine to the concentrations of the acrylamide monomer and bis-acrylamide.
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