To describe the impact of liver, gizzard, heart, spleen, pancreas in broilers meat production.My experimental broilers were reared for 35 days. After slaughtering offal's & glands were separately measured, but how can i compare the groups.
You can compare the relative (and absolute) weights of the organs between groups just as you can compare bodyweights, slaughter yields or evisceration yields between groups (see attached papers).
Article Evaluation of Broiler Performance when fed insect-protected,...
Article Evaluation of broiler performance when fed Roundup-Ready whe...
To better understanding the effects of such organs you said, it's better to consider them along with the management procedure or nutrition modification you did in your study.
As Dr. Kan mentioned you could compared weights between your treatments. But pay attention that weight increase or decrease are not good determiners of advantages or disadvantages of one treatment.
Definitely there is relation between body weight and these organ weight. To support th bigger body, definitely liver and other organs you mentioned have to work more which will cause to increase their weight. You can compare weights with treatments. Percent weight difference can be measure among control and treatment groups.
I also did some work to determine to effect of processed Jackfruit seeds on liver, gizzard, intestine, caecum, heart weights. The paper is attached for your consideration.
Article Ndyomugyenyi, E. K., Okot, M. W. and Mutetikka, D. (2015). T...
I often use weight of those organs as a percentage of empty carcass weight, rather than weights as it is. This will remove the compounding effects of higher body weights on organ weights.
It is also noted that digestive organs such as liver, intestine get priority in nutrient partitioning within the body. Therefore, we have observed higher wights of those organs when growth is retarded. Spleen weight is increased when there are anti-proteolytic substances in the diets.
We have to have the percentage weight of the mentioned organs to the body weight of normal chickens as the baseline first before we can compare to the treated groups. Remember, the weights of the organs cannot increase infinitely. There is a limit to everything
The answer depend on the hypothesis and the goals of your study, you can give more details about treatments and genetic groups, I hope the attached paper may be useful for you.