Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

How can I compare two calibration curves?

I have two calibration curves from sensors over different days. I want to compare the two calibration curves to one another. Is there way of doing this? The only ways I can think of doing this :...

17 August 2020 2,969 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to calculate flat membrane pore size distribution using membrane DI flux?

Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...

04 December 2019 3,850 2 View

Which part of polypeptide is susceptible for reaction?

A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!

17 October 2019 575 2 View

For many days enzyme expriment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced ?

For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?

01 September 2019 7,619 4 View

Subcloning of positive clone ?

I try to do subcloning of metagenomic DNA into Plasmid. The original DNA was in fosmid vector (PCC2Fos) with an insert size of nearly 40kb ( a positive clone enzymatic activity). The strategy I...

19 June 2019 171 3 View

No title

I look forward from hearing to you.

27 February 2019 3,197 3 View

Can someone help me to implement q-learning in multi user mobile edge computing in small cell with interferences?

HI EVERYBODY CAN SOMEONE help me to implement multi user computation offloading and resource allocation with mobile edge computing in small cell scenario where there are interference using...

13 November 2018 4,350 2 View

Can i used MTT to determine soil dehydrogenase?

In my understanding there are three main types of tetrazolium dyes that are used to determine dehydrogenase incluing MTT, INT, and TTC and for some reason i have encountered that MTT is rarely...

11 September 2018 3,590 2 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View