What is the resolution limit of DLS?

07 March 2024 1 2K Report

Can DLS measurement distinguis the hydrodynamic size of particles with 250nm and 270 nm? what is the resolution of the method?

Sergey Filippov

No. If the 250 and 270 particles are mixed together you can't resolve them independently. It will be one broad peak. As a rule of thumb, DLS can't resolve nanoparticles whose sizes differ by less than a factor of three.

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