Molecular ecology studies: in order to study fine-scale diversity in this species one needs to negate collection of clones.
I am calculating adsorption energies of molecules on surfaces using VASP. Every time I want to modify the starting configuration of the molecule, I am doing this long process of converting...
19 January 2021 2,624 3 View
My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...
20 July 2020 8,070 1 View
I am running jobs like # opt freq uwb97xd/6-311g(d,p) integral=grid=ultrafine geom=connectivity They complete the optimizations steps and then die during force calculations. An example of the...
08 July 2020 7,456 1 View
When running jobs similar to: # opt freq ub3lyp/6-311g geom=connectivity I often get this warning message: **** Warning!!: The largest alpha MO coefficient is 0.20327268D+02 Is this a...
13 May 2020 4,122 7 View
I'm looking into extracting DNA from buccal cells. I have no experience in using this source material and was wondering if there was a general consensus on preferred method? Yield would be a...
27 November 2019 240 3 View
Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative...
17 October 2019 3,713 4 View
I want to functionalize (3-Aminopropyl) triethoxysilane (APTES) on the surface of Mesoporous silica nanoparticles (MSN). Due to the availability of ethanol (99%) and magnetic stirrer, I took 0.5 g...
13 November 2018 9,942 7 View
I am synthesizing mesoporous silica nanoparticles and the procedure I performed in order to prepare mesoporous silica nanoparticles is as follows. 96.75 mL of double distilled water was taken in a...
26 August 2018 880 3 View
I have done phylogenetic analyses for both individual nuclear and mitochondrial markers- I ran the analyses with the correct model of substitution for each marker, appropriate outgroup and my...
06 March 2018 4,773 4 View
I am ideally looking for vignettes which describe dangerous crimes in a reasonable amount of detail, but without too much graphic detail, and where the evidence makes it unclear whether the...
04 December 2017 720 1 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...
26 February 2021 3,033 9 View
There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.
26 February 2021 5,851 1 View
For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...
16 February 2021 7,097 3 View
I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...
15 February 2021 3,642 6 View
There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...
11 February 2021 3,494 14 View
I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!
09 February 2021 1,419 8 View
I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.
04 February 2021 497 3 View
Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...
26 January 2021 4,278 4 View