I have worked on micropropagation of Taxus baccata and have some problem with propagation of this plant. The biggest problem is the yellowing of leaves. One or two weeks after culture the uppermost new leaves deteriorated and appeared yellowish green. then after 4 weeks explants completely died.
Almost all leaves desiccated and only the apical 6ud was alive with no development. The number of new shoot per explant is zero and only meristems grow up and then died.
I experience wide range of media and plant hormones but I could not solve these problems. I really need your help
Sincerely,
Possible causes include poor drainage, damaged roots, compacted roots, high soil pH, and nutrient deficiencies in the plant according to James Schuster University of Illinois Extension horticulture educato
Can you indicate your method of propagation? Is it via microcuttings, meristem culture or some other method. This would help to narrow down the cause(s) of the problems.
I think the yellowing of leaves due to the iron deficiency of culture medium, I expect your plant is sensitive for iron deficiency, so that your plant needs high concentration of iron.
thanks for response Mr Naveen but i do plant tissue culture. the problems you explain here are related to soil culture
Dear Amir
This yellowing and culture establishment problem is related to Iron uptake and it is also required higher concentration of H3BO3, NaMoO4, ZnSO4 and low level of Phytohormones.
Try to use below medium composition . You cal also modify this concentration and definitely get very good results.
Please use DKW medium + 2.5x of original DKW salts H3BO3 + 3x of original DKW salts NaMoO4.2H2O + add ZnSO4 65mg/L + add FeEDDHA 200mg/L (in Place of FeEDTA) + BAP 0.25 or 0.5mg/L
Regards
Do you check the temperature? warm condition during in vitro culture, may cause chlorosis symptoms.