I am trying to see the expression of Bim - BH3 only pro-apoptotic protein in dispersed mouse islet cells (seeded at 30,000 cells/well or 40,000 cells/well).
I have tried qPCR and western blot to check for the expression of Bim, but I haven't got any results. I am just wondering what is going wrong?
Thank you for your help in advance.
Is the antibody known to recognize mouse Bim?. Use a positive control to make sure it works.
Goodwin G. Jinesh , Thank you for your response. I have used a positive control for both western blot and qPCR. For qPCR I was able to see a considerable reduction in the C(t) values (increased expression of bim). However, still no expression in western blot. I am considering the no. of cells, probably is very low to detect protein levels of bim.
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