It is one of the biochemical identifications for Xanthomonas sp., where the pathogen cannot grow in 0.02% TTC and its growth is completely inhibited at 0.1% TTC.
As I know, most of Xanthomonads can be tolerant to 0.01% TTC only. Some old reports about xanthomonads growth on higher concentrations were about misidentified species, later placed to Pantoea.
Thank you for that sir . But can you say the mechanism on how it inhibits the growth of Xanthomonads .
TTC is a redox indicator commonly used in biochemical experiments especially indicate cellular respiration. TTC is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases. I guess it is a good competitor for usual bacterial substrates and reduce available enzymes activity.
See: https://www.researchgate.net/publication/259108332_Diminution_of_235-triphenyltetrazolium_chloride_toxicity_on_Listeria_monocytogenes_growth_by_iron_source_addition_to_the_culture_medium
Article Diminution of 2,3,5-triphenyltetrazolium chloride toxicity o...
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