I have a 96-well angiogenesis assay plate. For the first time, I will design an in vitro angiogenesis assay.
I'm going to test the potential for tube formation after treating the pancreatic cancer cells with a few drugs. I thought that pancreatic cancer cells would be seed directly into the wells and the potential for tube formation could be assessed. However, I have seen that HUVEC cells are used in the studies. Do I need to use HUVEC cells in this experiment? If yes, what is the reason for using it? Can you also tell us the tricks of the angiogenesis experiment?
Thanks for your help.
I have a similar business project, I'm waiting for someone to answer your question.
Angiogenesis is the process of making new blood vessels from existing ones. As tumor mass grows it becomes more hypoxic in its inner core. Then cancer cells secrete hypoxia inducing factor 1 (HIF1) that stimulates angiogenesis. HUVEC is a good choice because here the endothelial cells will proliferate to make a new blood vessel. Cancer cells can multiply, but they can not form a new tube.
Chandra Mohan Thank you for your answer.
So, do I need to co-culture pancreatic cancer cells with HUVEC to evaluate the angiogenesis potential of cancer cells?
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