The absorption spectra of retinal protein shows a noise near 580 nm? Is it noise or its inherent property? or it comes from the spectrophotometer?
What is the reason?
Thanks in Advance
Two major reasons for noisy spectra are an absorbance that is too high for the instrument to read properly and insolubility of the compound. The solution in both cases is to reduce the concentration of the compound.
Thank you Adam B Shapiro but this is not the issue of concentration or solubility. I checked it very carefully. I see this noise at high temperature (80 C). At low temperature no noise. And this noise is increasing with time during kinetic study.
Thank you İsmail Emir Akyildiz . I performed the blank analysis. No improvement.
Aggregation of self-assembly may shift the protein spectra and the known structure may be altered by heat treatment. Thereby, you should check your sample by using CD, or SEC-MALLS...
Have you ruled out air bubbles coming out of solution when it is heated, or convection?
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