grow cells overnight, extract protein, add glucose to it and incubate for 1 hour at 37 degree C. then do any test for glucose presence

Hi there,

Have a look at this, commercial kits are available for glucose/glucose oxydase detection using a coupled assay with peroxydase using a fluorometric method:

http://www.lifetechnologies.com/order/catalog/product/A22189

Hope it helps.

By the GOD-POD reagent method

Use a redox dye in your medium. If your organism is producing Glucose oxidase enzyme it will oxidise glucose to gluconic acid with simultaneous reduction in dye. Either a dye will change from colorless to colored or vice versa. You can also use pH indicator dyes such as bomocresol green, methyl red which change color when the medium changes from basic to acidic. Oxidation of glucose to gluconic acid will make your medium little acidic which can be easily tested using these dyes. Good luck

Dear Keerthivasan Chandradoss Raanin

Thanks for your answer but this is not for glucose test this is to detect Glucose oxidase presence. 

Dear Diwakar Pandey Please send the protocol for the GOD-POD reagent method on my e-mail [email protected].

Thanks

Please find the link below:

http://www.eplantscience.com/index/plant_protocols/carbohydrates/determination_of_glucose_by_glucose_oxidase.php